Effect Of Tetracycline on The Growth and Function of Autogenic Nitrogen-Fixing Bacteria

Xiuxuan Duan

Antibiotics, Biological nitrogen fixation, Nitrogen-fixing bacteria, Toxicity effects

The misuse of Tetracycline in agriculture has led to a significant increase and accumulation of residues in the soil environment. Tetracycline (TC) is one of the most commonly detected tetracycline antibiotics in recent years. To study the specific impact of antibiotics on soil microorganisms, this experiment used nitrogen-fixing bacteria as a biological model and co-cultured them with different concentrations of tetracycline medium. The co-culture products were then measured to record the growth, nitrogen fixation activity, morphological changes, and oxidative stress of the nitrogen-fixing bacteria. The results showed that tetracycline inhibited the growth, nitrogen fixation activity, and enzyme activity of nitrogen-fixing bacteria, with 2 mg/L of tetracycline and higher concentrations exhibiting stronger physiological and biochemical inhibitory effects on nitrogen-fixing bacteria. Our study indicates that Tetracycline are toxic to nitrogen-fixing microorganisms.

https://doi.org/10.31871/IJNTR.11.5.3