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International Journal of New Technology and Research

Impact Factor 3.953

(An ISO 9001:2008 Certified Online Journal)
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Comparison Between two Markers in the Identification of Free Plasma DNA Fractions in Pacients with Breast Cancer

( Volume 2 Issue 5,May 2016 ) OPEN ACCESS

Oliveira-Sediyama C. M. N., Abranches M. V., Sousa Moraes L. F., Freitas R. N., Ribeiro A.Q., Paula S. O., Peluzio M. C. G.


Cancer is one of the most cause of death in the world, with increased incidence each year. About 1.7 million women died In 2012, 1.7 million women died in the world with breast cancer. This study aimed to quantify the FDNAF by two markers, the gene expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and ß-actin, and evaluate which is the best to detect women with breast cancer.50 women were recruited to dose the FDNAF, 30 with breast cancer and 20 healthy controls. DNA was extracted from plasma, and Real time PCR reaction using the GAPDH and β-actin were performed. The GAPDH was the most adequate marker to identify the patients with breast cancer, because they presented higher levels of fragments of free DNA, when compared to healthy controls.

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