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Volume 1 Issue 3

S.No. Title & Authors Page No View
1

Title : Genetic Heterogeneity of 72 Patients With Mucopolysaccharidosis In Tunisia

Authors : Latifa Chkioua, Chaker Aloui, Sandrine Laradi, Oussama Grissa, Hadhami Ben Turkia, Souad Ouesleti, Salima Ferchichi, Abdelhedi Miled, Roseline Froissart

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The mucopolysaccharidoses (MPS) are a group of lysosomal storage diseases, presenting with a progressive multisystem disorder, with extreme clinical heterogeneity. The purpose of this study was to investigate six genes involved in different types of mucopolysaccharidosis (MPS), a group of lysosomal storage diseases. Mutation screening was performed for a total of 72 MPS Tunisian patients with MPS I (n=43), MPS II (n=5), MPS IIIA (n=7), MPS IIIB (n=3), MPS IIIC (n=2), or MPS IVA (n=12). The methodological approaches included genomic PCR sequencing, RT-PCR for MPS IVA and tetra-primer ARMS PCR assay for MPSI. The present study revealed one novel splice site mutation in one MPSI patient, in addition to the 11 previously Tunisian mutations reported for the first time by our research team: three in the IDUA gene (MPS I) including two missense, one nonsense; four in the SGSH gene (MPS IIIA) including a mutation involving the start codon, one small duplication, one small deletion and a large deletion of exons 1 to 5; two in the NAGLU gene (MPS IIIB) including one missense mutation and one nonsense mutation; two in the HGSNAT gene (MPS IIIC) including one missense and one nonsense mutation, two in the GALNS gene including one missense and one splicing mutation. These data demonstrate the remarkable mutational heterogeneity characterizing all types of MPS tested, although high consanguinity rate in the Tunisian population. We report biochemical and molecular aspects of these patients to prevent (e.g. genetic counseling, prenatal diagnosis) the recurrence of affected child since these diseases are still not rare in our area and as specific therapy is not available in our country.

 

01-06
2

Title : Antioxidant Activity and Total Phenolic and Flavonoid Contents of 30 Medicinal and Aromatic Plants Located in the South of Morocco

Authors : Ridouane El Guiche, Saida Tahrouch, Oukacha Amri, Khadija El Mehrach, Abdelhakim Hatimie

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In this study, antioxidant activity, total phenolic and total flavonoid contents of 30 species extracts, from 15 botanical families grown on three localities (Agdz, Tafraout and Taroudannt) of Souss Massa Draa (Southern Morocco), were determined using spectrophotometric methods. DPPH radical cation assay was used for evaluation of free radical scavenging properties of investigated species. The results showed a remarkable high antioxidant activity in the majority of tested plants, especially in Tetraclinis articulata, Thymus leptobotrys, and Lavandula stoechas, which neutralized up to 90 % of DPPH radicals. However, Conyza canadensis showed the lowest antioxidant value (88.19%). The total phenolics content measured by Folin-Ciocalteu method, ranged from 2.54 to 55.62 µg of gallic acid equivalents (GAE)/mg of dry matter (DM). Ceratonia siliqua (55.63 µg GAE /mg), Cistus villosus (41.10 µg GAE /mg), Limoniastrum feei (36.52 µg GAE /mg) and Rubus ulmifolius (35.02 µg GAE /mg) had very high levels of phenolics, whereas in Conyza canadensis (2.54 µg GAE /mg) phenolics were quit low. For total flavonoids content, two methods were used. Spectrophotmoetric method using NEU reagent showed a significant levels of flavonoids in Inula viscosa (70.08 µg/mg), Globularia alypum (66.28 µg/mg), Teucrium chamaedrys (63.95 µg/mg), Ruta montana (59.9 µg/mg) and Ononis natrix (55.49 µg/mg). For the second method with aluminium trichloride (AlCl3), the amounts of total flavonoid expressed as rutin equivalents/g DM, were higher in Rubus ulmifolius (117.79 µg/mg), Ononis natrix (80.84 µg/mg), Rhus pentaphulla (67.67 µg/mg), Thymus leptobotrys (53.17 µg/mg) and Thymus satureioides (52.34 µg/mg). According to this method Mentha pulegium exhibited the lowest flavonoids concentration (9.46 µg/mg), while the first method revealed low levels of flavonoids content in Conyza canadensis (5.41 µg/mg).

07-11
3

Title : Novel Peptide Suppresses Gene Expressions of C-MYC and CCND1 in Kasumi-1 Cell Lines

Authors : ChiyoMizuochi-Yanagi, Akane Yonehara, Yuka Tanaka, AnthonySwain, Toshiyuki Tsunoda, Senji Shirasawaand, Daisuke Sugiyama

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Leukemias are disorders that cause the abnormal growth and differentiation of hematopoietic cells. Therapies for leukemia are often invasive and there is no guarantee of a positive outcome. Biologically active peptides are being used for a number of therapies for different diseases. We derived several biologically active peptides from fetal liver derived Delta like-1+ hepatoblasts with the intention of finding novel biologically active peptides for the treatment of leukemia. After screening 9 peptides we discovered that two of the peptides caused changes of cell number in the human leukemic cell line Kasumi-1. Further investigation showed that a peptide with the sequence RRRRRRRR(PEG3)CQKKDGPCVINGS also caused suppression of the C-MYC and CCND1 genes at 1 day. The cell number and viability at 1 day along with the gene expression data suggests the peptide is a novel biologically active peptide that will be useful for the investigation of novel therapies for leukemia.

12-16
4

Title : Efficacy Study of Iodine Fortification of Milk on Iodine Status Markers: A Longitudinal Interventional, Controlled Study Among Schoolchildren in Morocco

Authors : Fatima Ezzahra Zahrou, Imane El Menchawy, Kaoutar Benjeddou, Issad Baddou, Khalid El Kari, Asmaa El Hamdouchi, Mohamed El Mzibri, El Arbi Bouaiti, Amina Barkat, El Arbi Rjimati, Hakim Belghiti, Noureddine El Haloui, Hassan Aguenaou

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Iodine deficiency is prevalent worldwide and can affect growth, mental development and learning ability of school children. In line with the Moroccan National Strategy for Nutrition 2011–2019, we undertook a study to evaluate  the efficacy of the consumption of iodine fortified milk on iodine status of children living in rural region. The stud is a longitudinal, interventional double-blinded (participants and assessors), controlled one concerning 200 children,  aged 7 to 9 years, recruited from 3 schools in a rural,  high altitude province in Morocco. The sample was divided in two groups: a non-fortified milk group (NFM, n=103) received 200ml of non-fortified Ultra High Temperature (UHT) milk and a fortified milk group (FM, n=50) received 200ml UHT milk fortified with iodine persulfate to cover 30%  of Recommended Daily Intake. Urine samples were collected at baseline, 4th and 9th month and urinary iodine was determined spectrophotometrically using the Sandell-Kolthoff reaction. Severe iodine deficiency was prevalent among the FM group. After interventions at 4th and 9th month, there was a marked improvement in the case of severe iodine deficiency in the FM group (4.0% at baseline, and none at 4th and 9th month).The NFM group had an important moderate decline from 3.9% at baseline to 1.9% at 9th month. For the moderate iodine deficiency, the prevalence was reduced from the baseline to the end of the study (9th month) ranging from 35.9% to 6.0% in both groups. Whereas for the mild iodine deficiency, the prevalence was increased at the 4th month  to 63.7% and 47.7% respectively in NFM and FM groups and then decreased at 9th month to 23.3% and 22.0% respectively in NFM and FM groups. The consumption of fortified UHT milk seems to be an effective strategy to reduce iodine deficiency among schoolchildren in this rural region of Morocco.

Trial registration: PACTR201410000896410. Registered 24 October 2014.

                              

17-24
5

Title : Organizational Hospital Management Team Working as Professional Technological Innovation

Authors : Nasser Fegh hi Farahmand, Nurse Nahideh Fegh hi Farahmand

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This paper proposes a relation of organizational hospital management team working by hospital management team working superintendence and reviews the organizational hospital management team working planning and performance measurement literature to develop a conceptual model and research propositions. Data are drawn from a survey of the comfort organizations in comfort organizations that around few samples of hospital management team working superintendence engage in organizational hospital management team working. The interview schedule was designed to collect data on a number of hospital management team working superintendence and strategic characteristics in addition to asking about the presence or absence of an organizational hospital management team working and, where appropriate, the time period to which the plan applied. Interviews were conducted face to face directly within the workplace, training and consultant sessions or indirectly by e-mail or using structured questionnaire. It is concluded that hospital management team working superintendence characteristics can be important in explaining and compilation the organizational hospital management team working within the comfort organizations for implementation of organizational hospital management team working planning. In fact, comfort organizations influence whether or not those organizations engage in organizational hospital management team working planning. In this field, the focus is on the special characteristics of hospital management team working superintendence such as education type and level.  

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6

Title : Understanding the Engineering Behavior of Soil from an Impact Crater, India

Authors : Channabasavaraj.W

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Lonar crater is an impact crater created by a meteor impact during the Pleistocene Epoch. Due to this, a lake called Lonar Lake, was formed, which lies in a basalt impact structure and is both saline and alkaline in nature. Geologists, ecologists, archaeologists, naturalists, and astronomers have reported several studies on various aspects associated with the Crater Lake ecosystem. Studies in the past are mainly focused on understanding the magnetic and gravitational effect of the crater. In addition, few attempts have been made by researchers to understand the characteristics (viz., chemical and morphological) of these soils. The current study focuses on complete characterization of the soils includes Physical, Chemical, Mineralogical and Morphological characteristics which is quite prudent in comprehending the geological history and external effects (viz., magnetic and gravitational effect of meteor impact) on these soils. It has been observed that the soil from the Lonar Crater is non-plastic to slightly plastic with high rate of swelling; also there is a presence of  a variety of heavy metals and minerals in the soil usually absent on the earth’s surface.

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7

Title : Augmenting the Properties of Black Cotton Soil Using Additives

Authors : Manjularani.P, Channabasavaraj.W, MdKhajaMoniuddin

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Black cotton soil is very susceptible to detrimental volume changes, with changes in moisture. This behavior of soil is attributed to the presence of mineral Montmorillonite which has expanding lattice; Black cotton soils because of their specific physical & chemical make are subjected to volume changes. In many countries including India, these soils are so extensive that alteration of highway route to avoid the material is virtually impossible. Various remedial measures like soil replacement, prewetting, moisture control, lime stabilization etc have been practiced with varied degree of success. Extensive research is going on to find the solutions to black cotton soils. In the present work experimentation is carried out to investigate efficacy of lime, fly ash, Alkalis and other Additives in improving the properties of black cotton soil.

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8

Title : Membrane Bioreactor Coupled with Sequential Batch Reactor: A Supportive Technology in Effluent Recycling Of API Manufacturing Industries

Authors : Srinivasarao Gangavarapu, Uma Maheswara Rao Ganguru, Sunil Kulkarni, Narasimha MurthyBrahmandam, Srinivasa Anumula

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Membrane Bioreactor (MBR) combined with Sequential Batch Reactor (SBR) was operated for treatment of wastewater generated in a medium scaleActive Pharmaceutical Ingredients (API) manufacturing unit. Parameters considered to investigate the performance of pilot plant wereChemical Oxygen Demand (COD), Biological Oxygen Demand (BOD) and Total Suspended Solids (TSS). However, to support the investigation, effluent Total Dissolved Solid (TDS) was also characterized. The studies indicated92.3 percent reduction in COD, 96.0 percent in BOD and 100 percent in TSS, and no significant effect on TDS reduction was found.The effluents streams taken up for study were characterized, neutralized, equalized and fed in SBR, which is a fill and draw activated sludge process driven by micro biological mass. The SBR out lets werereduced73.0, 79.9 and 76.3 percent by load in terms of COD, BOD and TSS respectively. The SBR outlets were fed in MBR, which is a membrane based reactor, where a further reduction of COD, BOD and TSS by72.0, 79.7 and 100 percent respectively was achieved. After successful pilot studies, it was found that the MBR combined with SBR can be operated positively for wastewater treatment in API manufacturing units, where effluent can be recycled after Reverse Osmosis (RO) polishing.

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